Siglec-3/CD33 Antibody (HIM3-4) [PE/Cy5.5]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-508PECY55

Clone HIM3-4 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB500-508PECY55
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Key Product Details

Species Reactivity

Human, Primate

Applications

Flow Cytometry

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # HIM3-4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Siglec-3/CD33 Antibodies »

Product Specifications

Immunogen

NFMY-9s human cell line

Localization

Cell Membrane

Specificity

The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. HLDA V; WS Code M MA112 HLDA VI; WS Code M MA47

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Siglec-3/CD33 Antibody (HIM3-4) [PE/Cy5.5]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Siglec-3/CD33

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody (HIM3-4) [PE/Cy5.5]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Siglec-3/CD33 Antibody (HIM3-4) [PE/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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