Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34511
![Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free [NBP2-34511]](https://resources.bio-techne.com/images/products/Siglec-3-CD33-Antibody-WM53-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34511-img0001.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free [NBP2-34511]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # WM53
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human AML cells
Localization
Cell surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32931).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free
Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free [NBP2-34511]
Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free [NBP2-34511] - Monocyte differentiation stages characterized by CD45, CD34, CD33 (NBP2-34511PECY7) and CD14 expression. HLA-DR and CCR2 expression were measured in each stage and expressed as the number of sites per cell. A) example of FACs analysis for monocyte differentiation (red: myelo/monoblasts, blue: promonocytes, green: monocytes), and the HLA-DR and CCR2 expression levels in each stage. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27893741/) licensed under a CC-BY license.Applications for Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Siglec-3/CD33
Long Name
Sialic Acid Binding Ig-like Lectin 3
Alternate Names
CD33, gp67, Siglec3
Gene Symbol
CD33
Additional Siglec-3/CD33 Products
Product Documents for Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free
Product Specific Notices for Siglec-3/CD33 Antibody (WM53) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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