Siglec-3/CD33 Antibody (WM53) [PE/Cy7]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34511PECY7
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry
Label
PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # WM53
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Human AML cells
Localization
Cell surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Siglec-3/CD33 Antibody (WM53) [PE/Cy7]
Flow Cytometry: Siglec-3/CD33 Antibody (WM53) [PE/Cy7] [NBP2-34511PECY7] - Monocyte differentiation stages characterized by CD45, CD34, CD33 (NBP2-34511PECY7) and CD14 expression. HLA-DR and CCR2 expression were measured in each stage and expressed as the number of sites per cell. A) example of FACs analysis for monocyte differentiation (red: myelo/monoblasts, blue: promonocytes, green: monocytes), and the HLA-DR and CCR2 expression levels in each stage. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27893741/) licensed under a CC-BY license.
Applications for Siglec-3/CD33 Antibody (WM53) [PE/Cy7]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Siglec-3/CD33
Long Name
Sialic Acid Binding Ig-like Lectin 3
Alternate Names
CD33, gp67, Siglec3
Gene Symbol
CD33
Additional Siglec-3/CD33 Products
Product Documents for Siglec-3/CD33 Antibody (WM53) [PE/Cy7]
Product Specific Notices for Siglec-3/CD33 Antibody (WM53) [PE/Cy7]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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