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STIM1 Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60547F

Novus Biologicals, part of Bio-Techne
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NB110-60547F

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine

Predicted:

Porcine (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

STIM1 antibody was made to a synthetic peptide from the C-terminal region (within residues 600-685) of the human STIM1 protein. [UniProt# Q13586]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: porcine (93%) and Xenopus (79%).

Localization

Cell membrane, ER membrane. Translocates from ER to cell membrane in response to depletion of intracellular calcium.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for STIM1 Antibody [FITC]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: STIM1

Stromal interaction molecule 1 (STIM1 or GOK) is a type 1 transmembrane protein that mediates store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores. STIM1 is Ca2+ sensor that conveys information about ER lumen's Ca2+ load to store-operated Ca2+ channels. It exists as a single transmembrane protein located in ER membrane with a luminal EF hand and a cytosolic domain, which activates Ca2+ release-activated Ca2+ channels. Upon Ca2+ depletion, STIM1 translocates from ER to plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/ORAI1 resulting in a strong Ca2+ influx. STIM1 is one of several genes located in imprinted gene domain of 11p15.5, an important tumor-suppressor gene region and alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. STIM1 implicates in early hematopoiesis by mediating attachment to stromal cells and defective STIM1 have been linked to immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.

Long Name

Stromal Interaction Molecule 1

Alternate Names

GOK

Gene Symbol

STIM1

Additional STIM1 Products

Product Documents for STIM1 Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for STIM1 Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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