TIM-1/KIM-1/HAVCR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56421

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Rat

Predicted:

Monkey (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TIM-1/KIM-1/HAVCR Antibodies »

Product Specifications

Immunogen

A synthetic peptide (SPLYSYTTDGNDTVT) of human TIM-1 protein was used as immunogen for this antibody. This sequence corresponds to amino acids (aa) 248-262 of NP_036338 and aa 84-98 of EAW61615.1. Isoforms of TIM-1 have been described. For example, NP_036338 is 364 aa and EAW61615.1 is 194 aa. EAW61615.1 is referred to as isoform CRA_a.

Reactivity Notes

Predicted to react with New World Monkey.

Specificity

Isoforms of different molecular weights have been described for TIM-1. The sequence used for immunogen is conserved between TIM-1 (364aa, NP_036338) and TIM-1 isoform CRA_a (194aa, EAW61615.1). Therefore, the molecular weight observed in western blots may vary depending on the forms present.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TIM-1/KIM-1/HAVCR Antibody

Western Blot: TIM-1/KIM-1/HAVCR Antibody [NB100-56421]

Western Blot: TIM-1/KIM-1/HAVCR Antibody [NB100-56421]

Western Blot: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Analysis of TIM-1/KIM-1/HAVCR in human brain lysate in the A) absence and B) presence of immunizing peptide, and C) mouse brain lysate using TIM-1 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody [NB100-56421]

Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody [NB100-56421]

Immunocytochemistry/Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody [NB100-56421] - Caco-2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-TIM-1/KIM-1/HAVCR Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Applications for TIM-1/KIM-1/HAVCR Antibody

Application
Recommended Usage

Western Blot

1-3 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIM-1/KIM-1/HAVCR

Official Gene Symbol HAVCR1 Gene ID 26762(Human) Gene Map Locus 5q33.2(Human) HAVCR1, a 359 A.A Class 1 integral membrane glycoprotein, is used by Hepatitis A virus to infect humans. It consists of a conserved N-terminal Cys-rich region, IgV (Immunoglobulin Variable region like) domain and a TSP-rich mucin domain in the extracellular region followed by a highly conserved tyrosine kinase phosphorylation motif in the cytoplasmic region. HAVCR1 is expressed on terminally differentiated CD4+ Th2 cells and regulates cytokine production. It plays an important role in the regulation of immune responses in the development of asthma and allergic responses. Its expression is critically upregulated in dedifferentiated kidney cells, thus indicating a possible role in regeneration of proximal tubule epithelium in Kidneys. HAVCR1 has shown to be upregulated in renal carcinomas, suggesting its role in tumor progression and metastasis.

Long Name

T Cell Immunoglobulin Mucin-1

Alternate Names

CD365, HAVCR1, KIM-1, TIM1

Entrez Gene IDs

26762 (Human); 171283 (Mouse)

Gene Symbol

HAVCR1

Additional TIM-1/KIM-1/HAVCR Products

Product Documents for TIM-1/KIM-1/HAVCR Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TIM-1/KIM-1/HAVCR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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