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Vanilloid R1/TRPV1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97417

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Invertebrate, Primate

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry (Negative), Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the rat TRPV1 protein (between residues 1-50)

Reactivity Notes

Reactive to Octopus vulgaris.

Localization

Multi-pass membrane protein. Note: Mostly, but not exclusively expressed in post-synaptic dendritic spines.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Vanilloid R1/TRPV1 Antibody - BSA Free

Western Blot: Vanilloid R1/TRPV1 AntibodyBSA Free [NBP1-97417]

Western Blot: Vanilloid R1/TRPV1 AntibodyBSA Free [NBP1-97417]

Western Blot: Vanilloid R1/TRPV1 Antibody [NBP1-97417] - Analysis of TRPV1 in following cell lysates: (1) Ntera2, (2) HepG2, (3) MCF7, and (4) Cos7.
Immunocytochemistry/ Immunofluorescence: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-97417]

Immunocytochemistry/ Immunofluorescence: Vanilloid R1/TRPV1 Antibody - BSA Free [NBP1-97417]

Immunocytochemistry/Immunofluorescence: Vanilloid R1/TRPV1 Antibody [NBP1-97417] - TRPV1 Antibody [NBP1-97417] - TRPV1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Applications for Vanilloid R1/TRPV1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:40

Western Blot

1:1000
Application Notes
In Western Blot, a band is seen at ~100 kDa representing TRPV1. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In ICC/IF, plasma membrane staining is observed in Ntera-2 cells. This antibody is not recommended for IHC-paraffin embedded sections.

Reviewed Applications

Read 1 review rated 5 using NBP1-97417 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vanilloid R1/TRPV1

TRPV1 (transient receptor potential cation channel subfamily V member 1, capsaicin receptor, vanilloid receptor, Vr1) is a polymodal nociceptor activated by heat, vanilloids (capsaicin), and protons. It is primarily expressed in the dorsal root ganglia and peripheral nerve endings. TRPV1 antagonists are a potential therapeutic target for many suffering chronic physical and neuropathic pain. However, due to its other role in the regulation of body temperature, these therapies are currently experiencing a barrier. On the other hand, TRPV1 agonists can be used to desensitize nerves after activation, thereby reducing pain. The research around TRPV1 is broad, where it is studied in fields such as anxiety, pain, oxidative stress, taste, inflammation, depression, respiratory disease, and various psychiatric disorders.

Long Name

Vanilloid Receptor 1/Transient Receptor Potential Cation Channel 1

Alternate Names

Capsaicin Receptor, TRPV1, VanilloidR1, VR1

Entrez Gene IDs

83810 (Rat)

Gene Symbol

TRPV1

UniProt

Additional Vanilloid R1/TRPV1 Products

Product Documents for Vanilloid R1/TRPV1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Vanilloid R1/TRPV1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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