VE-Cadherin Antibody (BV14) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43347CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-43347AF350

Alexa Fluor 405

NBP1-43347AF405

Alexa Fluor 488

NBP1-43347AF488

Alexa Fluor 532

NBP1-43347AF532

Alexa Fluor 594

NBP1-43347AF594

Alexa Fluor 647

NBP1-43347AF647

Alexa Fluor 700

NBP1-43347AF700

Alexa Fluor 750

NBP1-43347AF750

Biotin

NBP1-43347B

DyLight 350

NBP1-43347UV

DyLight 405

NBP1-43347V

DyLight 488

NBP1-43347G

DyLight 550

NBP1-43347R

DyLight 594

NBP1-43347DL594

DyLight 650

NBP1-43347C

DyLight 680

NBP1-43347FR

DyLight 755

NBP1-43347IR

FITC

NBP1-43347F

HRP

NBP1-43347H

Janelia Fluor 525

NBP1-43347JF525

Janelia Fluor 549

NBP1-43347JF549

Janelia Fluor 585

NBP1-43347JF585

Janelia Fluor 635

NBP1-43347JF635

Janelia Fluor 646

NBP1-43347JF646

Janelia Fluor 669

NBP1-43347JF669

mFluor Violet 450 SE

NBP1-43347MFV450

mFluor Violet 500 SE

NBP1-43347MFV500

mFluor Violet 610 SE

NBP1-43347MFV610

Forumulation

Catalog #

BSA Free

NBP1-43347

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Rat IgG_2B Clone # BV14

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VE-Cadherin Antibodies »

Product Summary for VE-Cadherin Antibody (BV14) [CoraFluor™ 1]

Immunogen

The immunogen for this antibody was CD144.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34179146).

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for VE-Cadherin Antibody (BV14) [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: VE-Cadherin

VE-cadherin (cadherin-5) is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. VE-cadherin is linked to the actin cytoskeleton through a number of adaptor proteins including a-, â-, and ã-catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The cytosolic C-terminal Src kinase (Csk) binds via its SH2 domain to the phosphorylated tyrosine 658 site of VE-cadherin. This interaction regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has also been shown to lead to the uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Gene Symbol

CDH5

Additional VE-Cadherin Products

Product Documents for VE-Cadherin Antibody (BV14) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for VE-Cadherin Antibody (BV14) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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