VE-Cadherin Antibody (BV14) [Janelia Fluor® 646]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43347JF646

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Janelia Fluor 646

Antibody Source

Monoclonal Rat IgG2B Clone # BV14

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VE-Cadherin Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was CD144.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34179146).

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for VE-Cadherin Antibody (BV14) [Janelia Fluor® 646]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: VE-Cadherin

VE-cadherin (cadherin-5) is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. VE-cadherin is linked to the actin cytoskeleton through a number of adaptor proteins including a-, â-, and ã-catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The cytosolic C-terminal Src kinase (Csk) binds via its SH2 domain to the phosphorylated tyrosine 658 site of VE-cadherin. This interaction regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has also been shown to lead to the uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Gene Symbol

CDH5

Additional VE-Cadherin Products

Product Documents for VE-Cadherin Antibody (BV14) [Janelia Fluor® 646]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VE-Cadherin Antibody (BV14) [Janelia Fluor® 646]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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