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Human IL-18/IL-1F4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA0561

Novus Biologicals, part of Bio-Techne
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KA0561

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatant, Cell Lysates, Plasma (EDTA, Heparin), Serum (100 uL)

Sensitivity

<1 pg/mL

Assay Range

15.6 to 1000 pg/mL

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

IL18 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human IL18.

Kit Contents for Human IL-18/IL-1F4 ELISA Kit (Colorimetric)

  • 96-well plate precoated with anti- human IL-18 antibody
  • ABC diluent buffer
  • Antibody diluent buffer
  • Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  • Biotinylated anti- human IL-18 antibody, dilution 1:100
  • Lyophilized recombinant human IL-18 standard
  • Sample diluent buffer
  • TMB color developing agent
  • TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-18/IL-1F4

The protein encoded by the IL18 gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. (provided by RefSeq)

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Gene Symbol

IL18

Additional IL-18/IL-1F4 Products

Product Documents for Human IL-18/IL-1F4 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-18/IL-1F4 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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