Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-94192

Novus Biologicals, part of Bio-Techne
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NBP1-94192
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

23.0 pg/mL

Assay Range

39.1 - 2,500 pg/mL

View all IL-18/IL-1F4 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Porcine

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a pig IL-18 positive serum. There was no crossreactivity detected

Description

The pig IL-18 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of pig IL-18

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.8%

Inter-Assay Precision (Precision between assays) CV% < 8.1%

Recovery for Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)

Recovery

The overall mean recovery was 110%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)

ELISA: Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric) [NBP1-94192]

Kit Contents for Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to pig IL-18
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-pig IL-18 monoclonal antibody
  • Pig IL-18 Standard lyophilized, 5 ng/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-18/IL-1F4

Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells. In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1. The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Gene Symbol

IL18

Additional IL-18/IL-1F4 Products

Product Documents for Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Porcine IL-18/IL-1F4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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