Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10509-GP
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived Glypican 3 protein
Gln25-Asn554, with a C-terminal 6-His tag
Gln25-Asn554, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln25 (blocked) & Ser359
Predicted Molecular Mass
38 kDa (alpha chain), 23 kDa (beta chain), 61 kDa (full length)
SDS-PAGE
65-90 kDa, under non-reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human FGF basic/FGF2 (146aa) (Catalog # 233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag (Catalog # 10509-GP) binds with an ED50 of 0.075-0.45 μg/mL.
When Recombinant Human FGF basic/FGF2 (146aa) (Catalog # 233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag (Catalog # 10509-GP) binds with an ED50 of 0.075-0.45 μg/mL.
Scientific Data Images for Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF
Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein Binding Activity
When Recombinant Human FGF basic/FGF2 (146 aa) Protein (233-FB/CF) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Glypican 3 His-tag Protein (10509-GP) binds with an ED50 of 0.075-0.45 μg/mL.Formulation, Preparation and Storage
10509-GP
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Glypican 3
References
- Ho, M. and Kim, H. (2012) Eur. J. Cancer. 47:333.
- Haruyama, Y. and Kataoka, H. (2016) World J. Gastroenterol. 22:275.
- Shimizu, Y. et al. (2019) Front Oncol. 9:248.
- Ge, S. et al. (2018) Filmus, Int J Clin Exp Pathol. 11(12):5774.
- Xue, Y. et al. (2018) Am J Pathol. 188(6):1469.
Alternate Names
GPC3
Gene Symbol
GPC3
UniProt
Additional Glypican 3 Products
Product Documents for Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF
Product Specific Notices for Recombinant Cynomolgus/Rhesus Glypican 3 His-tag Protein, CF
For research use only
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