Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera, CF

R&D Systems, part of Bio-Techne | Catalog # 9676-TM

R&D Systems, part of Bio-Techne
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9676-TM-100
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Key Product Details

Source

CHO

Accession #

BAJ61042

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TIM-1/KIM-1/HAVCR Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey TIM-1/KIM-1/HAVCR protein
Cynomolgus Monkey TIM-1/KIM-1/HAVCR
(Val18-Thr253)
Accession # BAJ61042		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val18, Asp20, & Val22

Predicted Molecular Mass

52 kDa

SDS-PAGE

91-107 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera, CF

Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera Bioactivity

Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera Bioactivity

Recombinant Cynomolgus Monkey TIM‑1/KIM‑1/HAVCR Fc Chimera (Catalog # 9676-TM) inhibits anti-CD3-induced proliferation of PHA-activated human T cells. The ED50 for this effect is 0.4-2.4 μg/mL.

Formulation, Preparation and Storage

9676-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

T cell immunoglobulin and mucin domain 1 (TIM-1), also known as KIM-1 and HAVcr1, is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses (1). TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain (2). Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain. TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule (3). TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (1, 4-6). It is up-regulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (7-10). Within the extracellular domain, Cynomolgus monkey TIM-1 shares 69% and 44% amino acid sequence identity with human and mouse TIM-1, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (2, 11). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (12). TIM-1 ligation induces T cell proliferation and promotes cytokine production (12).

References

  1. Du, P. et al. (2016) J. Immunol. Res. 2016:8605134.
  2. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  3. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  4. Ding, Q. et al. (2011) J. Clin. Invest. 121:3645.
  5. Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
  6. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
  7. Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
  8. Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
  9. Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
  10. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.

Long Name

T Cell Immunoglobulin Mucin-1

Alternate Names

CD365, HAVCR1, KIM-1, TIM1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Gene Symbol

HAVCR1

UniProt

BAJ61042

Additional TIM-1/KIM-1/HAVCR Products

Product Documents for Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera, CF

Product Datasheets

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Product Specific Notices for Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera, CF

For research use only

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