Recombinant Human Active SHP-1 (aa 205-595) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1878-SH
Key Product Details
Product Specifications
Source
E. coli-derived human SHP-1 protein
Ala205-Lys595, with an N-terminal Met and 6-His tag (C-terminal variants predicted)
Ala205-Lys595, with an N-terminal Met and 6-His tag (C-terminal variants predicted)
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
46 kDa
SDS-PAGE
40-46 kDa, reducing conditions
Activity
Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006).
The specific activity is >40 µmol/min/mg, as measured under the described conditions.
The specific activity is >40 µmol/min/mg, as measured under the described conditions.
Formulation, Preparation and Storage
1878-SH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: SHP-1
Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (Catalog # http://www.rndsystems.com/product_results.aspx?k=ES006">ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (Catalog # http://www.rndsystems.com/product_results.aspx?k=DY996">DY996).
References
- Tsui, H.W. et al. (1993) Nature Genet. 4:124.
- Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
- Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
- Pei, D. et al. (1994) Biochemistry 33:15483.
- Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.
Long Name
Src Homology Region 2 Domain Phosphatase 1
Alternate Names
HCPH, PTP1C, PTPN6, SHP1
Gene Symbol
PTPN6
UniProt
Additional SHP-1 Products
Product Documents for Recombinant Human Active SHP-1 (aa 205-595) Protein, CF
Product Specific Notices for Recombinant Human Active SHP-1 (aa 205-595) Protein, CF
For research use only
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