Recombinant Human Angiopoietin-like 4 Biotin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT8249

N-Terminal Fragment
R&D Systems, part of Bio-Techne
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BT8249-050
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Key Product Details

Source

CHO

Accession #

Q9BY76

Structure / Form

Oligomer. Biotinyated via sugars

Conjugate

Biotin

Applications

Bioactivity

View all Angiopoietin-like Protein 4/ANGPTL4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 4/ANGPTL4 protein
Gly26-Arg161, with 3xGSL and a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly26

Predicted Molecular Mass

17 kDa (unlabeled)

Activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL).
The ED50 for this effect is 25-125 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Scientific Data Images for Recombinant Human Angiopoietin-like 4 Biotin Protein, CF

Recombinant Human Angiopoietin-like 4 Biotin Protein Bioactivity

Recombinant Human Angiopoietin-like 4 Biotin Protein Bioactivity

Both Biotinylated recombinant human (rh) ANGPTL4 (Catalog # BT8249) and unlabeled rhANGPTL4 (Catalog # 8249-AN) stimulate the expansion of E16-E18 rat liver mononuclear cellsin vitro, in the presence of recombinant mouse (rm) SCF (Catalog # 455-MC), rmTpo (Catalog # 488-TO), and rmFlt-3L (Catalog # 427-FL). The ED50 for this effect is 25-125 ng/mL in the presence of a cross-linking antibody, His Tag MAb (Catalog # MAB050). The similarity in activity highlights that the biotinylated protein is fully functional.

Formulation, Preparation and Storage

BT8249
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Within the
N-terminal region, it shares approximately 67% aa sequence identity with mouse and rat ANGPTL4. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In human, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins beta1 and beta5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).

References

  1. Zhu, P. et al. (2012) Biosci. Rep. 32:211.
  2. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
  3. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
  4. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
  5. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
  6. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
  7. Cazes, A. et al. (2006) Circ. Res. 99:1207.
  8. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
  9. Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
  10. Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
  11. Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
  12. Hou, M. et al. (2014) PLoS ONE 9:e85808.
  13. Clement, L.C. et al. (2011) Nat. Med. 17:117.

Alternate Names

Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Gene Symbol

ANGPTL4

UniProt

Q9BY76

Additional Angiopoietin-like Protein 4/ANGPTL4 Products

Product Documents for Recombinant Human Angiopoietin-like 4 Biotin Protein, CF

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Product Specific Notices for Recombinant Human Angiopoietin-like 4 Biotin Protein, CF

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