Recombinant Human Angiopoietin-like 7 Biotin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT914

R&D Systems, part of Bio-Techne
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BT914
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Key Product Details

Source

CHO

Accession #

O43827

Structure / Form

Oligomer, Biotinylated on protein carbohydrates

Conjugate

Biotin

Applications

Bioactivity

View all Angiopoietin-like Protein 7/ANGPTL7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 7/ANGPTL7 protein
Gln27-Pro346, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results: Gln27 predicted, sequencing might be blocked

Predicted Molecular Mass

39 kDa (unlabeled)

SDS-PAGE

>110 kDa, reducing conditions

Activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL).
The ED50 for this effect is 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Scientific Data Images for Recombinant Human Angiopoietin-like 7 Biotin Protein, CF

Recombinant Human Angiopoietin-like 7 Biotin Protein Bioactivity

Recombinant Human Angiopoietin-like 7 Biotin Protein Bioactivity

Both Biotinylated rhANGPTL7 (Catalog # BT914) and unlabeled Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) stimulate the expansion of E16-E18 rat liver mononuclear cellsin vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) , and Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL). The ED50 for this effect is 50-200 ng/mL in the presence of a cross-linking antibody, His Tag MAb (Catalog # MAB050) . The similarity in activity highlights that the biotinylated protein is fully functional.

Formulation, Preparation and Storage

BT914
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 7/ANGPTL7

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1-3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45-50 kDa monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up-regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5-7). Over-expression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When over-expressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). ANGPTL7, when used in combination with SCF, Thrombopoietin, IGF-II, and FGF acidic, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (9).

References

  1. Oike, Y. et al. (2009) Circ. J. 73:2192.
  2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
  3. Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782.
  4. Peek, R. et al. (2002) J. Biol. Chem. 277:686.
  5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438.
  6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
  7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
  8. Comes, N. et al. (2010) Genes Cells 16:243.
  9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Long Name

Angiopoietin-like Protein 7

Alternate Names

ANGPTL7, AngX, CDT6

Entrez Gene IDs

10218 (Human); 654812 (Mouse); 102127960 (Cynomolgus Monkey); 714204 (Rhesus Macaque)

Gene Symbol

ANGPTL7

UniProt

O43827

Additional Angiopoietin-like Protein 7/ANGPTL7 Products

Product Documents for Recombinant Human Angiopoietin-like 7 Biotin Protein, CF

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Product Specific Notices for Recombinant Human Angiopoietin-like 7 Biotin Protein, CF

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