Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10494

Biotinylated
R&D Systems, part of Bio-Techne
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AVI10494-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

NP_001333870.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all EGFR Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human EGFR protein
Human EGFR Isoform vIII
(Leu25-Ser378)
Accession # NP_001333870.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

67 kDa

SDS-PAGE

95-115 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human EGFR Isoform vIII antibody (Novus Catalog # 50599) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human EGFR Isoform vIII Fc Chimera Avi-tag (Catalog # AVI10494)binds with an ED50 of 25-150 ng/mL.

Scientific Data Images for Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF

Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein Binding Activity

Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein Binding Activity

When Human EGFR Isoform vIII antibody (Novus Catalog # BNP-50599) is immobilized at 0.1 μg/mL (100 μL/well), Biotinylated Recombinant Human EGFR Isoform vIII Fc Chimera Avi-tag (AVI10494) binds with an ED50 of 25-150 ng/mL.
Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein SDS-PAGE

Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein (AVI10494) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 190-230 kDa, respectively.

Formulation, Preparation and Storage

AVI10494
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

Epidermal growth factor receptor (EGFR), also known as HER-1 and ErbB1, is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR, ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3‑4). EGFRvIII is a tumor‐specific mutation that results from an in‐frame deletion removing 267 amino acids from the ECD and insertion of a glycine residue (5). EGFRvIII has a molecular mass of approximately 145 kDa and has been shown to have weaker activity than full-length EGFR (6). EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7‑9). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (7-9). EGFR is overexpressed in a wide variety of tumors, with EGFRvIII overexpressed particularly in glioblastoma multiforme (GMB), and is the target of several anti-cancer therapeutics (5,10,11). Our Avi-tag Biotinylated Recombinant Human EGFRvIII features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Guillaudeau, A. et al. (2012) PLoS One. 7:1.
  4. Reiter J.L. et al. (2001) Genomics 71:1.
  5. Gan HK et al. (2013) FEBS J. 280:5350
  6. Batra SK, et al. (1995) Cell Growth Differ 6:1251
  7. Freed, D. M. et al. (2017) Cell. 171:683.
  8. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  9. Faria, J. A. et al. (2016) BBRC. 478:39.
  10. An Z. et al. (2018) Oncogene. 37:1561.
  11. Lee, C. K. et al. (2017) J. Thoracic Oncology. 12:403.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

NP_001333870.1

Additional EGFR Products

Product Documents for Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human EGFR Isoform vIII Fc Avi-tag Protein, CF

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