Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10468

Biotinylated Isoform V176
R&D Systems, part of Bio-Techne
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AVI10468-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

AAH17865.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Fc gamma RIIIA/CD16a Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein
Human Fc gamma RIIIA/CD16a
(Gly17-Gln208)
Accession # AAH17865.1		 6-His tag
 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly17

Predicted Molecular Mass

24 kDa

SDS-PAGE

48-57  kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When Human IgG is immobilized  at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human Fc gamma RIIIA/CD16a His-tag Avi-tag (Catalog # AVI10468) with an ED50 of 4-40 ng/mL.

Scientific Data Images for Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein, CF

Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein Binding Activity

Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein Binding Activity

When Human IgG is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human Fc gamma RIIIA/CD16a His-tag Avi-tag (AVI10468) with an ED50 of 4-40 ng/mL.
Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein SDS-PAGE

Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human Fc gamma RIIIA/CD16a His-tag Avi-tag (AVI10468) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-57 kDa.

Formulation, Preparation and Storage

AVI10468
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIIA/CD16a

Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes (1-3). In humans, it is expressed as a 50-70 kDa transmembrane activating receptor on NK cells, T cells, monocytes, and macrophages (2). It is closely related to the GPI-linked Fc gamma RIIIB which is expressed on human neutrophils and eosinophils (1, 3). These two proteins share 97% amino acid (aa) identity within their extracellular domains (ECD) (4). The ECD of Fc gamma RIIIA also shares 63%, 61%, 65%, 59%, and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIA, feline CD16, bovine CD16, and porcine Fc gamma RIIIB, respectively. Mature human Fc gamma RIIIA consists of a 192 aa ECD with two C2-type Ig-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (5, 6). Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma-chain or CD3 zeta (7, 8). Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA (9). Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity (10-13). Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis (13). Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases (11, 12). Our Avi-tag Biotinylated human Fc gamma RIIIA features biotinylation at a single site contained within the Avi-tag, a unique 15 aa peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
  2. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
  3. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
  4. Scallon, B.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5079.
  5. Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
  6. Dall’Ozzo, S. et al. (2004) Cancer Res. 64:4664.
  7. Kim, M.-K. et al. (2003) Blood 101:4479.
  8. Lanier, L.L. et al. (1989) Nature 342:803.
  9. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.    
  10. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
  11. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
  12. Masuda, M. et al. (2006) Atherosclerosis 188:377.
  13. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.

Long Name

Fc gamma Receptor III A

Alternate Names

CD16a, FCGR3A, FcgRIIIA

Entrez Gene IDs

2214 (Human); 304966 (Rat)

Gene Symbol

FCGR3A

UniProt

AAH17865.1

Additional Fc gamma RIIIA/CD16a Products

Product Documents for Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein, CF

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