Recombinant Human TGF-beta RII Protein
R&D Systems, part of Bio-Techne | Catalog # 241-R2
Key Product Details
Product Specifications
Source
Ile24-Asp159
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 1-3 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.
Reviewed Applications
Read 3 reviews rated 4.7 using 241-R2 in the following applications:
Scientific Data Images for Recombinant Human TGF-beta RII Protein
Bioactivity of Human TGF-beta RII
Recombinant human TGF-beta RII (241-R2) inhibits recombinant human TGF-beta 1 activity in the HT-2 mouse T cell line. The ED50 for this effect is 1-3 µg/mL in the presence of 0.25 ng/mL of recombinant human TGF-beta 1.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 241-R2
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 241-R2/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TGF-beta RII
Most cell types express three sizes of receptors for TGF-beta. These are designated type I (53 kDa), type II (70-85 kDa), and type III (250-350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.
The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.
References
- Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.
Long Name
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Gene Symbol
UniProt
Additional TGF-beta RII Products
Product Documents for Recombinant Human TGF-beta RII Protein
Product Specific Notices for Recombinant Human TGF-beta RII Protein
For research use only