Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9048-B7

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9048-B7-100
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q9EP73

Conjugate

Unconjugated

Applications

Bioactivity

View all PD-L1/B7-H1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PD-L1/B7-H1 protein
Phe19-His239, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe19

Predicted Molecular Mass

26 kDa

SDS-PAGE

49-62 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse PD-L1/B7-H1 is immobilized at 25 ng/mL (100 µL/well), the concentration of Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) that produces 50% of the optimal binding response is approximately 8-40 ng/mL

Reviewed Applications

Read 13 reviews rated 5 using 9048-B7 in the following applications:

Scientific Data Images for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Recombinant Mouse PD-L1/B7-H1 His-tag Protein Binding Activity

Recombinant Mouse PD-L1/B7-H1 His-tag Protein Binding Activity

When Recombinant Mouse PD-L1/B7-H1 is immobilize at 25 ng/mL, it binds Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) with an ED50 of 8-40 ng/mL.

Formulation, Preparation and Storage

9048-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Tamura, H. et al. (2001) Blood 97:1809.
  3. Chen, L. et al. (2007) J. Immunol. 178:6634.
  4. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  5. Tsushima, F. et al. (2007) Blood 110:180.
  6. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  7. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  8. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  9. Dong, H. et al. (2002) Nat. Med. 8:793.
  10. Azuma, T. et al. (2008) Blood 111:3635.
  11. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  12. Park, J.-J. et al. (2010) Blood 116:1291.
  13. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  14. Herold, M. et al. (2015) J. Immunol. 195:3584.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9EP73

Additional PD-L1/B7-H1 Products

Product Documents for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.