Recombinant Rat Insulysin/IDE Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6958-ZN

R&D Systems, part of Bio-Techne
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6958-ZN-010
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P35559

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Insulysin/IDE Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat Insulysin/IDE protein
Met42-Leu1019, with an N-terminal Met and 5-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

114 kDa

SDS-PAGE

95-115 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >1,600 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6958-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol, and Brij-35.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Insulysin/IDE

Insulysin, or insulin-degrading enzyme (IDE), is a zinc metallopeptidase of the inverzincin family. IDE is primarily located in the cytosol, but has been detected as a secreted enzyme and associated with the plasma membrane as well (1). The enzyme is expressed in many tissues, with the highest levels in liver, kidney, brain, and testis (2). IDE hydrolyzes a variety of regulatory peptides, including insulin, glucagon, atrial natriuretic factor, and transforming growth factor-a in vitro (1). In addition, IDE has been shown to degrade the amyloid b (Ab) peptide, which polymerizes into the plaques associated with Alzheimer’s disease (3). Deficiencies in IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus (DM2) and Alzheimer’s disease. The IDE region of human chromosome 10q has been genetically linked to DM2 (4). When the IDE gene was specifically disrupted in mice, IDE -/- animals developed hyperinsulinemia and glucose intolerance, characteristics of DM2 (5). The IDE -/- mice were also shown to have a significant decrease in Ab degradation in the brain, resulting in increased cerebral accumulation of Ab peptide (6). This in vivo evidence is consistent with the hypotheses that IDE is important for the degradation of insulin in cells and for the clearance of Ab peptide in the brain. Rat Insulysin displays 95.5% and 98.5 % sequence homology with human and mouse insulysin, respectively.

References

  1. Affholter, J. A. et al. (1988) Science 242:1415.
  2. Duckworth, W. C. et al. (1998) Endocr. Rev. 19:608.
  3. Akiyama, H. et al. (1990) Biochem. Biophys. Res. Commun. 170:1325.
  4. Selkoe, D. J. et al. (2001) Neuron 32:177.
  5. Ghosh, S. et al. (2000) Am. J. Hum. Genet. 67:1174.
  6. Farris, W. et al. (2003) Proc. Natl. Acad. Sci. 100:4162.

Long Name

Insulin Degrading Enzyme

Alternate Names

IDE, INSDEGM, Insulinase

Entrez Gene IDs

3416 (Human); 15925 (Mouse); 25700 (Rat)

Gene Symbol

IDE

UniProt

P35559

Additional Insulysin/IDE Products

Product Documents for Recombinant Rat Insulysin/IDE Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Rat Insulysin/IDE Protein, CF

For research use only

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