Rabbit IgG Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF008
Key Product Details
Species Reactivity
Rabbit
Applications
Simple Western, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Rabbit IgG
Specificity
Detects rabbit IgG heavy and light chains. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG and chicken IgY is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rabbit IgG Biotinylated Antibody
Detection of Rabbit IgG Antibody by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 24 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Rabbit IgG Antibody by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 71 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Rabbit IgG Biotinylated Antibody
Application
Recommended Usage
Simple Western
Use at a 1:500 dilution
Western Blot
Use at a 1:5000 dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using BAF008 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Rabbit IgG Biotinylated Antibody
Product Specific Notices for Rabbit IgG Biotinylated Antibody
For research use only
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