Advanced Neuroscience Research and Development with Simple Western

Advanced Neuroscience with the Most Data from Precious Samples

Some of the most prevalent and least understood diseases are those that affect the nervous system. Simple Western™ is winning the race to discovery in neuroscience research and development with high sensitivity multiplex protein assays at unprecedented speeds. Get the most data out of your precious sample. With Single-Cell Western on Milo™, you can measure heterogeneity in your samples and study protein expression within specific rare neural cell subtypes.

Advanced neuroscience research with Simple Western requires only 3 µL of sample while offering pg-level sensitivity, so there is no longer any need to pool samples like mouse tissue. Get single-animal statistical data and realize significant cost savings and save mouse lives. Simple Western also ups the data point count on laser capture microdissection (LCM) samples.

RePlex with Total Protein Detection

To get the most data out of precious samples like brain homogenate, Simple Western eliminates the arduous strip and re-probe procedure by offering RePlex™, which efficiently removes the antibodies from the first round of probing for a second round of probing with fresh antibodies. The second round may also be used for Total Protein Detection, so you can normalize your data with confidence.

Right Figure: Total protein concentration was used as the internal loading control for protein quantification in tau transgenic mice. (A-B) Simple Western analysis of brain homogenate from tau transgenic mice using antibodies against Lamin A/C and b-Actin. (C) Simple Western Total Protein assay detects total protein in each brain homogenate sample. (D) Quantification of Lamin A/C, (E) b-Actin and (F) total protein concentration. (G) Representative electropherogram generated from the Simple Western Total Protein assay. Figure adapted with permission from Musi et al. (2018) Aging Cell. 

Dissociation of Mouse Neural Tissue for Single-Cell Western Analysis

In this App Note, we describe and characterize a protocol to successfully dissociate mouse neural tissue micro-surgically dissected from combined cortex, ventricular zone, and hippocampus regions of E18 mice into single cells. Using a multiplexed Single-Cell Western assay to analyze protein expression in the dissociated single neuronal cells, we identify 3 subpopulations of cells within the tissue: astrocytes, radial glia, and immature neurons based on expression of their respective phenotypic protein markers: GFAP, Pax6, and β-III tubulin.