Human PIAS3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5120
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PIAS3
Met10-Asp628
Accession # Q9Y6X2
Met10-Asp628
Accession # Q9Y6X2
Specificity
Detects human PIAS3 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PIAS3 Antibody
Detection of Human PIAS3 by Western Blot.
Western blot shows nuclear extracts of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PIAS3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5120) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PIAS3 at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PIAS3 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF-7 human breast cancer cell line
Sample: HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF-7 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIAS3
PIAS3 (protein inhibitor of activated STAT protein 3) is a 65-75 kDa member of the PIAS family of proteins. It is a ubiquitously expressed nuclear E3-type SUMO ligase that binds to, and inhibits, the gene activating activity of STAT3, MITF and the progesterone receptor. Human PIAS3 is 628 amino acids (aa) in length and contains a SAP domain (aa 11‑45) with an embedded transcriptional coregulator LxxLL motif, a STAT3 binding site (aa 82‑132), a PINIT motif required for nuclear retention (aa 241‑245), an SP-RING-type zinc finger domain (aa 303‑380) and a SUMO1-binding region (aa 450‑460). There is an alternate start site at Met10. Human PIAS3 is 97% aa identical to mouse and canine PIAS3 over both aa 1‑628 and 10‑628.
Long Name
Protein Inhibitor of Activated STAT 3
Alternate Names
ZMIZ5
Gene Symbol
PIAS3
UniProt
Additional PIAS3 Products
Product Documents for Human PIAS3 Antibody
Product Specific Notices for Human PIAS3 Antibody
For research use only
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