PIAS3 Antibody (4F12)

Novus Biologicals, part of Bio-Techne | Catalog # H00010401-M03

Novus Biologicals, part of Bio-Techne
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H00010401-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIAS3 Antibodies »

Product Specifications

Immunogen

PIAS3 (NP_006090, 453 a.a. ~ 550 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPTKKHCSVTSAAIPALPGSKGVLTSGHQPSSVLRSPAMGTLGGDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDALGHF

Reactivity Notes

Other species not tested.

Specificity

PIAS3 (4F12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PIAS3 Antibody (4F12)

Western Blot: PIAS3 Antibody (4F12) [H00010401-M03]

Western Blot: PIAS3 Antibody (4F12) [H00010401-M03]

Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Analysis of PIAS3 expression in transfected 293T cell line by PIAS3 monoclonal antibody (M03), clone 4F12. Lane 1: PIAS3 transfected lysatE (67 KDa). Lane 2: Non-transfected lysate.
Western Blot: PIAS3 Antibody (4F12) [H00010401-M03]

Western Blot: PIAS3 Antibody (4F12) [H00010401-M03]

Western Blot: PIAS3 Antibody (4F12) [H00010401-M03] - Western blot analysis of PIAS3 over-expressed 293 cell line, cotransfected with PIAS3 Validated Chimera RNAi or non-transfected control. Blot probed with H00010401-M03. GAPDH (36.1 kDa) used as loading control.

Applications for PIAS3 Antibody (4F12)

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIAS3

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined.

Long Name

Protein Inhibitor of Activated STAT 3

Alternate Names

ZMIZ5

Entrez Gene IDs

10401 (Human)

Gene Symbol

PIAS3

OMIM

605987 (Human)

UniProt

NP_006090 (Human)

Additional PIAS3 Products

Product Documents for PIAS3 Antibody (4F12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIAS3 Antibody (4F12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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