MUC5AC Antibody (45M1)

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196]

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] -

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.