Biotin Antibody [Texas Red]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-3647
Key Product Details
Species Reactivity
Non-species specific
Applications
Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot
Label
Texas Red (Excitation = 589 nm, Emission = 615 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotinylated bovine serum albumin and biotinylated IgG.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotinylated bovine serum albumin and biotinylated IgG.
Scientific Data Images for Biotin Antibody [Texas Red]
Western Blot: Biotin Antibody [Texas Red] [NB110-3647] - Lane 1: Biotin conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Guinea Pig IgG. Other band(s): None.
Applications for Biotin Antibody [Texas Red]
Application
Recommended Usage
Fluorophore-linked immunosorbent assay
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:500-1:500
Western Blot
1:100-1:2000
Application Notes
This product has been tested by dot blot and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Biotin
Long Name
Biotin
Alternate Names
Vitamin B4
Additional Biotin Products
Product Documents for Biotin Antibody [Texas Red]
Product Specific Notices for Biotin Antibody [Texas Red]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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