Biotin Antibody [Rhodamine] - F(ab')2
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97383
Key Product Details
Species Reactivity
Non-species specific
Applications
Immunocytochemistry/ Immunofluorescence, Knockout Validated
Label
Rhodamine (Excitation = 570 nm, Emission = 590 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Red Excitation Wavelength: 550 Emission Wavelength: 570
Conjugation Chemistry: Isothiocyanate
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Biotin conjugated IgG
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Red Excitation Wavelength: 550 Emission Wavelength: 570
Conjugation Chemistry: Isothiocyanate
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Biotin conjugated IgG
Applications for Biotin Antibody [Rhodamine] - F(ab')2
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500-1:2500
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Biotin
Long Name
Biotin
Alternate Names
Vitamin B4
Additional Biotin Products
Product Documents for Biotin Antibody [Rhodamine] - F(ab')2
Product Specific Notices for Biotin Antibody [Rhodamine] - F(ab')2
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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