Biotin Antibody [FITC] - F(ab)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97372
Key Product Details
Species Reactivity
Non-species specific
Applications
Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Green Excitation Wavelength: 495 Emission Wavelength: 528
Conjugation Chemistry: Isothiocyanate
Fab fragment anti-Biotin Antibody [FITC]was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
Color: Green Excitation Wavelength: 495 Emission Wavelength: 528
Conjugation Chemistry: Isothiocyanate
Fab fragment anti-Biotin Antibody [FITC]was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein
Scientific Data Images for Biotin Antibody [FITC] - F(ab)
Biotin Antibody [FITC] - F(ab)
Dot Blot results of Goat Biotin Antibody [FITC] - F(ab). Dots top row: Biotin-BSA Conjugate. Dots bottom row: BSA alone. Load: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Fab Anti-Biotin Antibody FITC at 1ug/mL in 1hr RT. Imaged with BioRad ChemiDoc, Fluorescein filter.Applications for Biotin Antibody [FITC] - F(ab)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:500-1:2500
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:20-1:50
Western Blot
1:100-1:500
Application Notes
This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Biotin
Long Name
Biotin
Alternate Names
Vitamin B4
Additional Biotin Products
Product Documents for Biotin Antibody [FITC] - F(ab)
Product Specific Notices for Biotin Antibody [FITC] - F(ab)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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