DOK2 Antibody (OTI1A4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00948

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A4

Concentration

1 mg/ml

View all DOK2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DOK2 (NP_003965) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DOK2 Antibody (OTI1A4)

Western Blot: DOK2 Antibody (OTI1A4) [NBP2-00948]

Western Blot: DOK2 Antibody (OTI1A4) [NBP2-00948]

Western Blot: DOK2 Antibody (1A4) [NBP2-00948] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK2.
Immunocytochemistry/Immunofluorescence: DOK2 Antibody (1A4) [NBP2-00948] Staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK2.
Flow Cytometry: DOK2 Antibody (OTI1A4) [NBP2-00948]

Flow Cytometry: DOK2 Antibody (OTI1A4) [NBP2-00948]

Flow Cytometry: DOK2 Antibody (1A4) [NBP2-00948] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DOK2 antibody, and then analyzed by flow cytometry.

Applications for DOK2 Antibody (OTI1A4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DOK2

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq].

Alternate Names

docking protein 2, docking protein 2, 56kD, docking protein 2, 56kDa, Dok-2, Downstream of tyrosine kinase 2, p56(dok-2), P56DOK, p56dok-2

Entrez Gene IDs

9046 (Human)

Gene Symbol

DOK2

Additional DOK2 Products

Product Documents for DOK2 Antibody (OTI1A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DOK2 Antibody (OTI1A4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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