Histone H2AX [p Ser139] Antibody (RM224)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18533

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM224

Concentration

1 mg/ml

View all Histone H2AX Antibodies »

Product Specifications

Immunogen

A phospho-peptide corresponding to Histone H2AX [Ser139]

Modification

p Ser139

Specificity

This antibody reacts to Histone H2AX only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2AX [p Ser139] Antibody (RM224)

Western Blot: Histone H2AX [p Ser 119] Antibody (RM224) [NBP3-18533] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using NBP3-18533 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.
Immunocytochemistry/Immunofluorescence: Histone H2AX [p Ser 119] Antibody (RM224) [NBP3-18533] - Immunocytochemistry of HeLa cells using NBP3-18533 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Applications for Histone H2AX [p Ser139] Antibody (RM224)

Application
Recommended Usage

ELISA

0.2 ug/mL - 1 ug/mL

Immunocytochemistry/ Immunofluorescence

0.5 ug/mL - 2 ug/mL

Multiplex Immunoassay

0.1 ug/mL - 1 ug/mL

Western Blot

0.5 ug/mL - 2 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX [p Ser139] Antibody (RM224)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2AX [p Ser139] Antibody (RM224)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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