Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26030
Recombinant Monoclonal Antibody
![Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26030_rabbit-histone-h2ax-mab-rm224-azide-and-bsa-free-22620242311083.png "Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] -")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Vertebrate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM224
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free
Immunogen
A phospho-peptide corresponding to Histone H2AX [Ser139]
Specificity
This antibody reacts to Histone H2AX only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] -
Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] - Immunocytochemistry of HeLa cells using Clone RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).![Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26030_rabbit-histone-h2ax-mab-rm224-azide-and-bsa-free-24620241332456.png "Western Blot: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] -")
Western Blot: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] -
Western Blot: Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free [NBP3-26030] - Western Blot of acid extracts of HeLa cells treated or non-treated with UV, using RM224 at 0.5 ug/mL, showed a band of Histone H2AX phosphorylated at serine 139 in HeLa cells.Applications for Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.2 ug/mL - 1 ug/mL
Immunocytochemistry/ Immunofluorescence
0.5 ug/mL - 2 ug/mL
Multiplex Immunoassay
0.1 ug/mL - 1 ug/mL
Western Blot
0.5 ug/mL - 2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2AX
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free
Product Specific Notices for Histone H2AX [p Ser139] Antibody (RM224) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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