Human Galectin-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5447
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Galectin-10
Ser2-Arg142
Accession # Q05315
Ser2-Arg142
Accession # Q05315
Specificity
Detects human Galectin-10 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Galectin-10 Antibody
Detection of Human Galectin‑10 by Western Blot.
Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Galectin‑10 in Human Tregs by Flow Cytometry.
Human Tregs were expanded from PBMC using Cloudz Human Treg Expansion Kit (CLD006) then stained with (A) Goat Anti-Human Galectin-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) or (B) Goat IgG Control Antibody (AB-108-C) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107) and Mouse Anti-Human CD25/IL-2 Ra Allophycocyanin-conjugated Monoclonal Antibody (FAB1020A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.
Detection of Galectin‑10 in Human PBMC lymphocytes by Flow Cytometry.
Human PBMC lymphocytes were stained with Goat Anti-Human Galectin-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) followed by CFS-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109) and Mouse Anti-Human CD25/IL-2 Ra Allophycocyanin-conjugated Monoclonal Antibody (Catalog # FAB1020A).Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).Applications for Human Galectin-10 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Human Tregs or PBMC lymphocytes fixed with paraformaldehyde and permeabilized with saponin
Sample: Human Tregs or PBMC lymphocytes fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: HL-60 human acute promyelocytic leukemia cell line
Sample: HL-60 human acute promyelocytic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-10
Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one Galectin domain (aa 6‑138) that contains two dimerization motifs (aa 6‑10 and 131‑135). Two molecular weight isoforms of 15 and 14 kDa have been described that are not yet characterized. There is no known structural rodent counterpart to human Galectin-10.
Alternate Names
CLC, GAL10, Galectin10, LGALS10
Entrez Gene IDs
1178 (Human)
Gene Symbol
CLC
UniProt
Additional Galectin-10 Products
Product Documents for Human Galectin-10 Antibody
Product Specific Notices for Human Galectin-10 Antibody
For research use only
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