Human Integrin alpha2b/CD41 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7616
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
ELISA Detection, Interferent Testing, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 745201
Product Specifications
Immunogen
heterodimer of human Integrin alpha 2B (Leu32-Arg993; R887L) Accession P08514 + human Integrin beta 3 (Gly27-Asp718) Accession P05106
Specificity
Detects human Integrin alpha2b/CD41 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human Integrin alpha5, alpha8, alphaV, beta3, beta5, beta6, recombinant mouse Integrin beta2b or beta3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Integrin alpha2b/CD41 Antibody
Detection of Human Integrin alpha2b/CD41 by Western Blot.
Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin a2b/CD41 Monoclonal Antibody (Catalog # MAB7616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Integrin a2b/CD41 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Integrin alpha2b/CD41 in Human Blood Platelets by Flow Cytometry.
Human peripheral blood platelets were stained with Fluorescein-conjugated Anti-Mouse Integrin a2b/CD41 (Clone P2) and either (A) Mouse IgG1Isotype Control (MAB002) or (B) Mouse Anti-Human Integrin a2b/CD41 Mono-clonal Antibody (Catalog # MAB7616) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). MAB7616 stains the same population as Clone P2.
Detection of Integrin alpha 2b/CD41 in Human Blood Platelets by Flow Cytometry.
Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 Mono-clonal Antibody (Catalog # MAB7616, filled histogram) or Mouse IgG1 Isotype Control (MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.Applications for Human Integrin alpha2b/CD41 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood platelets
Sample: Human peripheral blood platelets
Western Blot
2 µg/mL
Sample: Human platelets
Sample: Human platelets
Reviewed Applications
Read 1 review rated 5 using MAB7616 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 2b/CD41
Alternate Names
CD41, GP2B, GPIIb, GTA, HPA3, ITGA2b
Gene Symbol
ITGA2B
Additional Integrin alpha 2b/CD41 Products
Product Documents for Human Integrin alpha2b/CD41 Antibody
Product Specific Notices for Human Integrin alpha2b/CD41 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...