Human LAMP-1/CD107a Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC4800G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Intracellular Staining by Flow Cytometry
Cited:
Immunocytochemistry
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 508921
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP-1/CD107a
Ala28-Asn380
Accession # P11279
Ala28-Asn380
Accession # P11279
Specificity
Detects human LAMP1/CD107a in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LAMP-1/CD107a Alexa Fluor® 488-conjugated Antibody
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.
Human NK Cells either (A) stimulated in a 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 µM monensin for 5 hours or (B) ustimulated were stained with Mouse Anti-Human LAMP-1/CD107a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC4800G) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (FAB2408P). Quadrant markers were set based on control antibody staining (IC0041G). View our protocol for Staining Membrane-associated Proteins.
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.
Human NK Cells were expanded from PBMCs following the protocol on ExCellerate Human NK Cell Expansion Media, Xeno Free (CCM032) plus Recombinant Human IL-2 (27 ng/mL), Recombinant Human IL-12 (10 ng/mL), Recombinant Human IL-18 (10 ng/mL), and Recombinant Human IL-21 (10 ng/mL) for 14 days, then stained with (A) Mouse Anti-Human LAMP-1/CD107a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC4800G) or (B) Mouse IgG2B isotype control antibody (IC0041G) and Mouse Anti-Human NCAM-1/CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R). View our protocol for Staining Membrane-associated Proteins.
Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC4800G, filled histogram) or isotype control antibody (IC0041G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.Applications for Human LAMP-1/CD107a Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
5 µL/106 cells
Sample: Enriched human NK cells incubated with K562 human chronic myelogenous leukemia cell line target cells and stimulated with monensin, or Human NK Cells expanded from PBMCs in ExCellerate Human NK Cell Expansion Media, Xeno Free (Catalog # CCM032) plus Recombinant Human IL-2 (27 ng/mL), Recombinant Human IL-12 (10 ng/mL), Recombinant Human IL-18 (10 ng/mL), and Recombinant Human IL-21 (10 ng/mL) for 14 days,
Sample: Enriched human NK cells incubated with K562 human chronic myelogenous leukemia cell line target cells and stimulated with monensin, or Human NK Cells expanded from PBMCs in ExCellerate Human NK Cell Expansion Media, Xeno Free (Catalog # CCM032) plus Recombinant Human IL-2 (27 ng/mL), Recombinant Human IL-12 (10 ng/mL), Recombinant Human IL-18 (10 ng/mL), and Recombinant Human IL-21 (10 ng/mL) for 14 days,
Intracellular Staining by Flow Cytometry
5 µL/106 cells
Sample: THP-1 human acute monocytic leukemia cell line
Sample: THP-1 human acute monocytic leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAMP-1/CD107a
Long Name
Lysosome-associated Membrane Glycoprotein 1
Alternate Names
CD107a, LAMP1
Gene Symbol
LAMP1
UniProt
Additional LAMP-1/CD107a Products
Product Specific Notices for Human LAMP-1/CD107a Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...