Human LAMP-1/CD107a PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC4800P
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 508921
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
Ala28-Asn380
Accession # P11279
Ala28-Asn380
Accession # P11279
Specificity
Detects human LAMP1/CD107a in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LAMP-1/CD107a PE-conjugated Antibody
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.
NK cells were expanded from PBMC using Cloudz Human NK Cell Expansion Kit (CLD004) and cultured in the presence of Mouse Anti-Human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Catalog # IC4800P) for 2 hours either (A) with or (B) without a 1:1 ratio of K562 cell line target cells. Cells were then stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (FAB2408A). Quadrant markers were set based on control antibody staining (IC0041P). Staining was performed using our Staining Surface Molecules protocol.
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.
Enriched human NK cells either (A) treated with 1:1 ratio with K562 cell line for 5 hours or (B) untreated were stained with Mouse Anti-Human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Catalog # IC4800P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P).
Detection of LAMP‑1/CD107a in THP‑1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a PE-conjugated Monoclonal Antibody (Catalog # IC4800P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human LAMP-1/CD107a PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Enriched or expanded human NK cells cultured with 1:1 ratio of K562 cell line target cells
Sample: Enriched or expanded human NK cells cultured with 1:1 ratio of K562 cell line target cells
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: LAMP-1/CD107a
Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.
Long Name
Lysosome-associated Membrane Glycoprotein 1
Alternate Names
CD107a, LAMP1
Gene Symbol
LAMP1
UniProt
Additional LAMP-1/CD107a Products
Product Documents for Human LAMP-1/CD107a PE-conjugated Antibody
Product Specific Notices for Human LAMP-1/CD107a PE-conjugated Antibody
For research use only
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