Human NK Cells either (A) stimulated in a 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 µM monensin for 5 hours or (B) unstimulated were stained with Mouse Anti-Human LAMP-1/CD107a APC-conjugated Monoclonal Antibody (Catalog # IC4800A) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Detection of LAMP-1/CD107a in THP‑1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a APC-conjugated Monoclonal Antibody (Catalog # IC4800A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human LAMP-1/CD107a APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Enriched human NK cells incubated with K562 human chronic myelogenous leukemia cell line target cells

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: THP‑1 human acute monocytic leukemia cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAMP-1/CD107a

Lysosome-Associated Membrane Protein-1 (LAMP-1), also known as CD107a, is a 100-130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP-2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP-1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28-381) and a 12 aa cytoplasmic tail (aa 405-416). The ECD has two large looping regions (aa 28-193 and 227-381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28-380, human LAMP-1 shares 64% aa identity with mouse LAMP-1.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Gene Symbol

LAMP1

UniProt

P11279

Additional LAMP-1/CD107a Products

Product Documents for Human LAMP-1/CD107a APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human LAMP-1/CD107a APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human LAMP-1/CD107a APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC4800A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LAMP-1/CD107a Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human LAMP-1/CD107a APC-conjugated Antibody

Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry.