Human LAMP1/CD107a Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM4800
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2B Clone # 508921
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAMP1/CD107a
Ala28-Asn380
Accession # P11279
Ala28-Asn380
Accession # P11279
Specificity
LAMP1 antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. Detects human LAMP1/CD107a in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human LAMP1/CD107a Biotinylated Antibody
LAMP1/CD107a in HeLa Human Cell Line.
LAMP1/CD107a was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LAMP1/CD107a Biotinylated Monoclonal Antibody (Catalog # BAM4800) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human LAMP1/CD107a Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line.
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAMP-1/CD107a
Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.
Long Name
Lysosome-associated Membrane Glycoprotein 1
Alternate Names
CD107a, LAMP1
Gene Symbol
LAMP1
UniProt
Additional LAMP-1/CD107a Products
Product Documents for Human LAMP1/CD107a Biotinylated Antibody
Product Specific Notices for Human LAMP1/CD107a Biotinylated Antibody
For research use only
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