LAMP1 antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. Detects human LAMP1/CD107a in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human LAMP1/CD107a Biotinylated Antibody

LAMP1/CD107a in HeLa Human Cell Line.

LAMP1/CD107a was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LAMP1/CD107a Biotinylated Monoclonal Antibody (Catalog # BAM4800) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human LAMP1/CD107a Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Formaldehyde fixed HeLa human cervical epithelial carcinoma cell line.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAMP-1/CD107a

Lysosome-associated membrane protein-1 (LAMP1), also known as CD107a, is a 100‑130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T-cells, and with LAMP2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28‑381) and a 12 aa cytoplasmic tail (aa 405‑416). The ECD has two large looping regions (aa 28‑193 and 227‑381) plus multiple N- and O-linked glycosylation sites. There is one potential splice variant that shows a 26 aa substitution in the signal sequence. Over aa 28‑380, human LAMP1 shares 64% aa identity with mouse LAMP1.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Gene Symbol

LAMP1

UniProt

P11279

Additional LAMP-1/CD107a Products

Product Documents for Human LAMP1/CD107a Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human LAMP1/CD107a Biotinylated Antibody

For research use only

Citations
Reviews

Human LAMP1/CD107a Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM4800

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all LAMP-1/CD107a Antibodies »

Product Specifications

Immunogen

Specificity