Western blot shows lysates of U937 human histiocytic lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 15 minutes or 2 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 20 minutes, respectively. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-STAT6 (Y641) Monoclonal Antibody (Catalog # MAB3717) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-STAT6 (Y641) in Daudi Human Cell Line.

STAT6 phosphorylated at Y641 was detected in immersion fixed Daudi human Burkitt's lymphoma cell line, untreated (lower panel) or treated with Recombinant Human IL-4 (Catalog # 204-IL; upper panel) using Rabbit Anti-Human Phospho-STAT6 (Y641) Monoclonal Antibody (Catalog # MAB3717) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of STAT6 in Human peripheral blood mononuclear cells (PBMCs) by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or (B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) Monoclonal Antibody (Catalog # MAB3717) followed by anti-Rabbit IgG PE-conjugated secondary antibody (Catalog # F0110) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Applications for Human Phospho-STAT6 (Y641) Antibody

Application

Recommended Usage

Immunocytochemistry

2-10 µg/mL
Sample: Immersion fixed Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IL-4 (Catalog # 204-IL)

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Th2-stimulated human peripheral blood mononuclear cells (PBMCs) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol

Western Blot

1 µg/mL
Sample: U937 human histiocytic lymphoma cell line and HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human IL‑4 (Catalog # 204-IL)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN₄GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Long Name

Signal Transducer and Activator of Transcription 6

Alternate Names

D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT

Entrez Gene IDs

6778 (Human); 20852 (Mouse); 362896 (Rat)

Gene Symbol

STAT6

UniProt

P42226

Additional STAT6 Products

Product Documents for Human Phospho-STAT6 (Y641) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Phospho-STAT6 (Y641) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Phospho-STAT6 (Y641) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3717

Key Product Details

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Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

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Host

Isotype

Scientific Data Images for Human Phospho-STAT6 (Y641) Antibody

Detection of Human Phospho-STAT6 by Western Blot.