Mouse CXCR2/IL-8RB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2164
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
CyTOF-reported, Flow Cytometry, Immunohistochemistry, Neutralization
Cited:
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunohistochemistry-Frozen, In vivo assay, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 242216
Product Specifications
Immunogen
C6 rat glioma cell line transfected with mouse CXCR2
Met1-Leu359
Accession # P35343
Met1-Leu359
Accession # P35343
Specificity
Stains mouse CXCR2 transfected
cells but not CXCR1 transfectants.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CXCR2/IL-8RB Antibody
Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).CXCR2/IL‑8 RB in Mouse Spleen.
CXCR2/IL-8 RB was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell sufaces in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCR2/IL-8 RB Antibody.
Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164). The ND50 is typically 15-50 µg/mL.Applications for Mouse CXCR2/IL-8RB Antibody
Application
Recommended Usage
CyTOF-reported
Wang, G. et al. (2016) Cancer Discov. 6: 80. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CXCR2/IL-8 RB and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with mouse CXCR2/IL-8 RB and eGFP
Immunohistochemistry
5-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spleen
Sample: Perfusion fixed frozen sections of mouse spleen
Neutralization
Measured by its ability to neutralize CXCL2/GRO beta/MIP-2/CINC-3-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with mouse CXCR2. The Neutralization Dose (ND50) is typically 15-50 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta/MIP-2/CINC-3.
Reviewed Applications
Read 1 review rated 5 using MAB2164 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR2/IL-8RB
CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.
Long Name
Interleukin 8 Receptor B
Alternate Names
CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB
Gene Symbol
CXCR2
UniProt
Additional CXCR2/IL-8RB Products
Product Documents for Mouse CXCR2/IL-8RB Antibody
Product Specific Notices for Mouse CXCR2/IL-8RB Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...