Mouse gp130 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM4681
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry
Cited:
Flow Cytometry
Label
Biotin
Antibody Source
Monoclonal Rat IgG2A Clone # 125623
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse gp130
Met1-Glu617
Accession # Q00560
Met1-Glu617
Accession # Q00560
Specificity
Detects mouse gp130. No cross-reactivity with recombinant rat gp130 or recombinant human gp130 was observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse gp130 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: M1 mouse myeloid leukemia cell line
Sample: M1 mouse myeloid leukemia cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: gp130
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.
Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.
References
- Narazaki, M. et al. (1993) Blood 82:1120.
- Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Alternate Names
CD130, IL6ST
Gene Symbol
IL6ST
UniProt
Additional gp130 Products
Product Documents for Mouse gp130 Biotinylated Antibody
Product Specific Notices for Mouse gp130 Biotinylated Antibody
For research use only
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