Mouse Ly-6G/Ly-6C (Gr-1) Antibody

Ly-6G/Ly-6C (Gr-1) in Mouse Spleno-cytes.

Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed mouse splenocytes using Mouse Ly-6G/Ly-6C (Gr-1) Monoclonal Anti-body (Catalog # MAB1037) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.

Ly-6G/Ly-6C (Gr-1) was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Monoclonal Antibody (Catalog # MAB1037) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Mouse Ly-6G/Ly-6C (Gr-1) Antibody by Immunohistochemistry

Lymphocyte/neutrophil ratio increases upon NHE1 inhibition in tumor sections of KPfC mice.(A) H&E (left) and PAS-stained KPfC mouse tissue sections after vehicle and gemcitabine + cariporide (GEM+CARI) therapy. Cells of innate immunity, such as neutrophils (arrows), utilize glycogen and are thus PAS+ (purple), in contrast to, for example, lymphocytes. Scale bar: 50 μm. (B) Representative IHC images stained for Ly6G+ neutrophils (magenta, arrows on left image), CD3+ lymphocytes (yellow, arrows on the right image), and nuclei with DAPI (cyan). Scale bar: 50 μm. (C) CD3/Ly6G ratio was assessed by dividing the number of CD3+ cells by the number of Ly6G+ cells in every tumor node. Data points depict the mean CD3/Ly6G ratio derived from each tumor node in individual mice; NVehicle = 10, NGEM = 9, NCARI = 10, NGEM+CARI = 11 mice. (D) To obtain the CD3/Ly6G ratio per tumor node, the number of CD3+ cells was divided by the respective number of Ly6G+ cells in each tumor node. Data points depict individual tumor nodes; nVehicle = 386, nGEM = 276, nCARI = 301, nGEM+CARI = 398. Data and statistical comparison in D and E are represented as median ± 95% CI using Kruskal-Wallis statistical test with Dunn’s post hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/37643024), licensed under a CC-BY license. Not internally tested by R&D Systems.