Mouse Gr-1/Ly-6G PerCP-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1037C

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Functional Assay

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation = 482 and 564 nm, Emission = 675 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # RB6-8C5

View all Ly-6G/Ly-6C (Gr-1) Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody is Ly-6G (Gr-1, Gr1).

Specificity

Detects mouse Gr-1/Ly-6G. Weak cross-reactivity with Ly-6C is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Gr-1/Ly-6G PerCP-conjugated Antibody

Detection of Gr-1/Ly-6G antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of Gr‑1/Ly‑6G in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse Gr-1/Ly-6G PerCP-conjugated Monoclonal Antibody (Catalog # FAB1037C) or (B) Rat IgG2BPerCP Isotype Control (Catalog # IC013C). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Gr-1/Ly-6G PerCP-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References

  1. Spangrude, G.J. et al. (1988) Science 241:58.
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
  3. Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
  4. Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Ly-6C, Ly-6G, Ly6G

Entrez Gene IDs

546644 (Mouse)

Gene Symbol

LY6G

Additional Ly-6G/Ly-6C (Gr-1) Products

Product Documents for Mouse Gr-1/Ly-6G PerCP-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Gr-1/Ly-6G PerCP-conjugated Antibody

For research use only

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