Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) or (B) Rat IgG_2BFluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Ly-6G/Ly-6C (Gr-1)

The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

References

Spangrude, G.J. et al. (1988) Science 241:58.
Fleming, T.J. et al. (1993) J. Immunol. 151:2399.
Lewinsohn, D.M. et al.(1987) J. Immunol. 147:22.
Lagasse, E. and I.L. Weissman (1996) J. Immunol. Methods 197:139.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Ly-6C, Ly-6G, Ly6G

Entrez Gene IDs

546644 (Mouse)

Gene Symbol

LY6G

Additional Ly-6G/Ly-6C (Gr-1) Products

Product Documents for Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1037F

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Ly-6G/Ly-6C (Gr-1) Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Gr-1/Ly-6G Fluorescein-conjugated Antibody

Detection of Gr‑1/Ly‑6G in Mouse Splenocytes by Flow Cytometry.