NHE3/SLC9A3 [p Ser552] Antibody (14D5)
Novus Biologicals, part of Bio-Techne | Catalog # NB110-81529
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Opossum, Primate, Rabbit
Cited:
Human, Mouse, Rat, Porcine, Opossum, Primate
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Knockout Validated, Simple Western, Western Blot
Cited:
ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Knockout Validated, SDS-Page, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 14D5
Concentration
2 mg/ml
Product Specifications
Immunogen
Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]
Reactivity Notes
Porcine reactivity reported in scientific literature (PMID: 29510506).
Modification
p Ser552
Specificity
This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NHE3/SLC9A3 [p Ser552] Antibody (14D5)
Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.
Immunohistochemistry: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of verified customer review.
Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Applications for NHE3/SLC9A3 [p Ser552] Antibody (14D5)
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
reported in scientific literature (PMID 21255452)
Immunocytochemistry/ Immunofluorescence
1:50-1:1000
Immunohistochemistry
1:50-1:1000
Immunoprecipitation
1:10-1:500
Knockdown Validated
reported in scientific literature (PMID 31276916)
Knockout Validated
reported in scientific literature (PMID 31276916)
Simple Western
1:1000
Western Blot
1-5 ug/ml
Application Notes
In Western Blot, a band can be seen at approx. 85 kDa.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 review rated 4 using NB110-81529 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NHE3/SLC9A3
Long Name
Sodium/Hydrogen Exchanger 3
Alternate Names
SLC9A3
Gene Symbol
SLC9A3
Additional NHE3/SLC9A3 Products
Product Documents for NHE3/SLC9A3 [p Ser552] Antibody (14D5)
Product Specific Notices for NHE3/SLC9A3 [p Ser552] Antibody (14D5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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