NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 549]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-81529JF549

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Opossum, Primate, Rabbit

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Knockout Validated, Simple Western, Western Blot

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG2A Clone # 14D5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NHE3/SLC9A3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Specificity

This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 549]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NHE3/SLC9A3

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Long Name

Sodium/Hydrogen Exchanger 3

Alternate Names

SLC9A3

Gene Symbol

SLC9A3

Additional NHE3/SLC9A3 Products

Product Documents for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 549]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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