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NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Alexa Fluor® 350]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-81529AF350

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Opossum, Primate, Rabbit

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Knockdown Validated, Knockout Validated, Simple Western, Western Blot

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 14D5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide made to a region surrounding the Serine 552 portion of rat NHE3. [UniProt# P26433]

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Specificity

This antibody detects NHE3 posphorylated at Serine 552, and does not cross-react with the non-phosphopeptide.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Alexa Fluor® 350]

NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Alexa Fluor® 350]

NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Alexa Fluor® 350] [NB110-81529AF350] -

NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Alexa Fluor® 350] [NB110-81529AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.

Applications for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Alexa Fluor® 350]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NHE3/SLC9A3

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Long Name

Sodium/Hydrogen Exchanger 3

Alternate Names

SLC9A3

Gene Symbol

SLC9A3

Additional NHE3/SLC9A3 Products

Product Documents for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Alexa Fluor® 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NHE3/SLC9A3 [p Ser552] Antibody (14D5) [Alexa Fluor® 350]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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