Rat gp130 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5029

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Rat

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 745314

View all gp130 Antibodies »

Product Summary for Rat gp130 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat gp130
Gln23-Glu618 (predicted)
Accession # P40190

Specificity

Detects rat gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human or recombinant mouse gp130 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rat gp130 Antibody

Detection of Rat gp130 antibody by Western Blot.

Detection of Rat gp130 by Western Blot.

Western blot shows lysates of NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat gp130 Monoclonal Antibody (Catalog # MAB5029) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gp130 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of gp130 antibody in CD3+ Rat Splenocytes antibody by Flow Cytometry.

Detection of gp130 in CD3+ Rat Splenocytes by Flow Cytometry.

CD3+ rat splenocytes were stained with Mouse Anti-Rat gp130 Monoclonal Antibody (Catalog # MAB5029, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Rat gp130 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: CD3+ rat splenocytes

Western Blot

2 µg/mL
Sample: NRK rat normal kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gp130

Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Rat gp130 shares 88% and 79% aa sequence identity with mouse and human gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)‑6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2-5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).

References

  1. Wang, Y. et al. (1992) Genomics 14:666.
  2. Muller-Newen, G. (2003) Sci. STKE pe40.
  3. Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
  4. Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
  5. Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.

Long Name

Glycoprotein 130

Alternate Names

CD130, IL6ST

Entrez Gene IDs

3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)

Gene Symbol

IL6ST

UniProt

P40190 (Rat)

Additional gp130 Products

Product Documents for Rat gp130 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat gp130 Antibody

For research use only

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