Recombinant Mouse Angiopoietin-like 3 (aa 17-220) Biotin, CF

R&D Systems, part of Bio-Techne | Catalog # BT8344

N-Terminal Fragment
R&D Systems, part of Bio-Techne
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BT8344-050
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Key Product Details

Source

CHO

Accession #

Q9R182

Structure / Form

Biotinylated protein via sugars

Conjugate

Biotin

Applications

Bioactivity

View all Angiopoietin-like Protein 3/ANGPTL3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Angiopoietin-like Protein 3/ANGPTL3 protein
Ser17-Thr206, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser17

Predicted Molecular Mass

23 kDa (unlabeled)

Activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt-3 Ligand (Catalog # 427-FL).
The ED50 for this effect is 20-120 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Scientific Data Images for Recombinant Mouse Angiopoietin-like 3 (aa 17-220) Biotin, CF

Recombinant Mouse Angiopoietin-like 3 (aa 17-220) Biotin Bioactivity

Recombinant Mouse Angiopoietin-like 3 (aa 17-220) Biotin Bioactivity

Both Biotinylated rmANGPTL3 (Catalog # BT8344) and unlabeled rmANGPTL3 (Catalog # 8344-AN) stimulate the expansion of E16-E18 rat liver mononuclear cells link, in the presence of rmSCF (Catalog # 455-MC), rmTpo (Catalog # 488-TO), and rmFlt-3L (Catalog # 427-FL). The ED50 for this effect is 20-120 ng/mL in the presence of a cross-linking antibody, His Tag MAb (Catalog # MAB050). The similarity in activity highlights that the biotinylated protein is fully functional.

Formulation, Preparation and Storage

BT8344
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and CHAPS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like Protein 3 (ANGPTL3) is a secreted glycoprotein that is structurally related to the angiopoietins (1). Mature mouse ANGPTL3 contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (2). Mature mouse ANGPTL3 shares 77% aa sequence identity with human ANGPTL3. ANGPTL3 is expressed in the liver from early in development through adulthood (2, 3). Full length ANGPTL3 circulates in the plasma as do the proteolytically separated
N- and C-terminal fragments containing the coiled coil domain and fibrinogen-like domains, respectively (4, 5). The cleavage of ANGPTL3 by Furin and Proprotein Convertase 5/6 is enhanced by its interaction with the related ANGPTL8 (6, 7). ANGPTL3 is found as 70 kDa, 50 kDa, and 32 kDa species and can form weakly associated noncovalent multimers in vitro (3, 4). ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids (8, 9). This activity requires a putative heparin-binding motif which is N-terminal to the coiled coil domain (4). Proteolytic removal of the fibrinogen-like domain from the N-terminal fragment serves to activate ANGPTL3 and increase its ability to inhibit LPL in vitro and function in vivo [Ono 41804]. ANGPTL3 promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake (4, 5, 8). ANGPTL3 knockout mice are hypolipidemic and have elevated LPL activity (10). ANGPTL3 expression in vivo is upregulated by LXR agonists and downregulated by insulin, leptin, and agonists of TR beta or PPAR beta (11-14). Dysregulated ANGPTL3 expression and elevated plasma triglyceride levels are characteristic of some strains of obese and diabetic mice (5, 8, 12). ANGPTL3 does not bind Tie1 or Tie2, but its fibrinogen-like domain interacts with Integrin alphaV beta3 to induce endothelial cell adhesion, migration, and neovascularization (15). ANGPTL3, secreted by fetal liver cells, also promotes the expansion of hematopoietic stem cells (16).

References

  1. Santulli, G. (2014) Front. Endocrinol. (Lausanne) 5:4.
  2. Conklin, D. et al. (1999) Genomics 62:477.
  3. Ge, H. et al. (2005) J. Lipid Res. 46:1484.
  4. Ono, M. et al. (2003) J. Biol. Chem. 278:41804.
  5. Koishi, R. et al. (2002) Nat. Genet. 30:151.
  6. Essalmani, R. et al. (2013) J. Biol. Chem. 288:26410.
  7. Quagliarini, F. et al. (2012) Proc. Natl. Acad. Sci. USA 109:19751.
  8. Shimizugawa, T. et al. (2002) J. Biol. Chem. 277:33742.
  9. Shimamura, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:366.
  10. Koster, A. et al. (2005) Endocrinology 146:4943.
  11. Inaba, T. et al. (2003) J. Biol. Chem. 278:21344.
  12. Shimamura, M. et al. (2004) Biochem. Biophys. Res. Commun. 322:1080.
  13. Fugier, C. et al. (2006) J. Biol. Chem. 281:11553.
  14. Matsusue, K. et al. (2006) Mol. Cell Endocrinol. 256:23.
  15. Camenisch, G. et al. (2002) J. Biol. Chem. 277:17281.
  16. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

UniProt

Q9R182 (Mouse)

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Product Documents for Recombinant Mouse Angiopoietin-like 3 (aa 17-220) Biotin, CF

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