Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

Novus Biologicals, part of Bio-Techne | Catalog # KA4132

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Biological Samples (50 uL)

Sensitivity

0.1 mU/mL

Assay Range

0.1 to 100 mU/mL

View all Acetylcholinesterase/ACHE Activity Assays »

Product Specifications

Assay Type

Fluorometric

Kit Type

Assay Kit (Fluorometric)

Reactivity

Rat

Specificity

The kit is an optimized 'mix and read' assay that provides a simple one- step fluorimetric assay to detect as little as 0.01 mU AChE in a 100 uL assay volume (0.1 mU/mL). Its signal can be easily read with a fluorescence microplate reader at Ex/Em = ~540/590 nm or an absorbance microplate reader at ~575 nm.

Description

Quality control test: Standard curve Acetylcholinesterase Assay Kit (Red Fluorescence) is a one-step fluorometric assay for the detection of AChE activity.

Scientific Data Images for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

Acetylcholinesterase/ACHE Assay Kit (Fluorometric) [KA4132] - The standard curve is for the purpose of demonstration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

  • Acetylcholine
  • Acetylcholinesterase Probe (lyophilized powder)
  • Acetylcholinesterase Standard (5 units)
  • Amplite Red
  • Assay Buffer
  • Dilution Buffer
  • DMSO

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Acetylcholinesterase/ACHE

Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer's disease (AD) and myasthenia gravis.Acetylcholinesterase (AChE) is one of the most crucial enzymes for nerve response and function. AChE degrades the neurotransmitter acetylcholine (ACh) into choline and acetic acid. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate the synaptic transmission. AChE inhibitors are among the key drugs approved for Alzheimer's disease (AD) and myasthenia gravis.

Alternate Names

ACHE, ARACHE, N-ACHE

Gene Symbol

ACHE

Additional Acetylcholinesterase/ACHE Products

Product Documents for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Acetylcholinesterase/ACHE Assay Kit (Fluorometric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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