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Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24471

Novus Biologicals, part of Bio-Techne
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NBP3-24471

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, animal tissue and cell samples

Sensitivity

0.03 U/L

Assay Range

0.03 - 1.5 U/L

Product Specifications

Kit Type

Activity Assay Kit (Fluorometric)

Reactivity

Non-species specific

Description

Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/587 nm)
Assay time: 80 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 2.2

Inter-Assay Precision (Precision between assays) %CV < 7.3

Recovery for Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

Recovery

Average recovery rate: 100%

Scientific Data Images for Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

Aspartate Aminotransferase Activity Assay Kit (Fluorometric) [NBP3-24471] -

Aspartate Aminotransferase Activity Assay Kit (Fluorometric) [NBP3-24471] - Standard Reference Curve
Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

Aspartate Aminotransferase Activity Assay Kit (Fluorometric) [NBP3-24471] -

Aspartate Aminotransferase Activity Assay Kit (Fluorometric) [NBP3-24471] - Assay Example

Kit Contents for Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

  • Black Microplate
  • Plate Sealer
  • Reagent 1: Buffer Solution
  • Reagent 2: Substrate
  • Reagent 3: Enzyme Reagent
  • Reagent 4: Aqueous Alkali
  • Reagent 5: 100 mmol/L Pyruvate Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Aspartate Aminotransferase

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Alternate Names

aspartate aminotransferase, cytoplasmic, ASTQTL1, CASPAT, CCAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, EC 2.6.1.1, EC 2.6.1.3, GIG18, Glutamate oxaloacetate transaminase 1, glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1), GOT1, growth-inhibiting protein 18, Transaminase A

Gene Symbol

GOT1

Additional Aspartate Aminotransferase Products

Product Documents for Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Aspartate Aminotransferase Activity Assay Kit (Fluorometric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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